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Samstag, 09 Oktober 2004 |
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Laboratory Facilities 
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| Safety bench (radioactivity)
| Comet - Assay
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| Cell culture bench
| Isolated Lung perfusion
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| High performance liquid chromatography
| Gas chromatography FID, ECD
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| Inhalation Chambers
| Real-time PCR
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Lab working place
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Letzte Aktualisierung ( Samstag, 09 Oktober 2004 )
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Dissertations and Current Projects |
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Samstag, 09 Oktober 2004 |
Dissertations and Current Projects
- Dissertations 2001
- Enrico Weidauer: The influence of oxidative stress on the expression of antioxidative enzymes and aspects of the mechanism of toxicity of Paraquat
- Christoph Köhler
Expression of MDR1, MRP1 and LRP in human tumor cell lines and in normal human bronchial epitelial cells
- Thomas Lehmann
The adaption of the expression of MDR, MRP1 and LRP in primary cultures of human lung cells and tumor cell lines in culture
- Alice Rachidi
Determination of expression and inducibility of the cytochrome P450-isoforms 2E1, 1A1 and 2B in rat lung [Martin-Luther-University Medical faculty]
- Thomas Stock
Culture of normal human bronchial epithelial cells Markus Ebke: Pharmacokinetics of Prilocain in the perfusion of isolated lung and liver of rats
- Dissertations 2003
- Thilo Seidel
A survey of Risk factors for patients suffering from bladder carcinomas living in an industrial region in Saxony-Anhalt Current Projects
- Anja Rämisch
Sensitivity of lung cells to EGF/HGF and its possible function
- Abdel-Rahman Torky
Functional activity of the MDR-/MRP-transport proteins in human lung cells in vitro
- Felix Glahn
Regulation of MRP-proteins in human lung cells Isabel Thoms: Interaction of Dichloromethane, Ethanol and model compounds for genotoxicity in human lung cells - application of COMET Sabine Walcher: Genotoxicity of Vinylchloride in normal human bronchial epithelial cells - mechanism of interactions with other compounds
Current Projects
- Anja Rämisch
Sensitivity of lung cells to EGF/HGF and its possible function
- Abdel-Rahman Torky
Functional activity of the MDR-/MRP-transport proteins in human lung cells in vitro
- Felix Glahn
Regulation of MRP-proteins in human lung cells
- Isabel Thoms
Interaction of Dichloromethane, Ethanol and model compounds for genotoxicity in human lung cells - application of COMET
- Sabine Walcher: Genotoxicity of Vinylchloride in normal human bronchial epithelial cells - mechanism of interactions with other compounds
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Letzte Aktualisierung ( Sonntag, 10 Oktober 2004 )
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Samstag, 09 Oktober 2004 |
Other Activities
- "Umweltprobenbank Münster"
Cooperation with the human
tissue bank for monitoring of environmental contaminants in humans,
initiated and maintained by the University of Münster
- UTOXIS (Umwelttoxikologisches Informationssystem)
a cooperation between High
schools and the University Handling and interpretation of data on
environmental contaminations Initiation of experimental projects in
schools
- Member of the Universitätszentrum für
Umweltwissenschaften UZU
(university center for environmental sciences)
Initiation and coordination of interdisciplinary projects on
environmental topics and problems Risk perception
awareness socio-economical aspects management
of mega site pollutions sustainable use and resources Management
- Clinical Toxicology and Environmental Health Toxicological expertises:
Advising of institutions (governmental courts), clinical staff
(University) and in rare cases also private persons
- National
activities
S c i e n t i f i c s o c i e t i e s : Chairman, Section
Toxicology, German
Society of Pharmacology and Toxicology (DGPT)
G o v
e r n m e n t a l : Member of the German Advisory Council on the
Environment
Member of the advisory committee on drug safety (BfArm,
Kommission A)
Member of the advisory committee on intoxication and
chemical safety
(BgVV)
Member of the Committee on children and
juveniles (Kommission D)
Non go v e r nme n t a l : Member of the
advisory committee on chemicals
(BUA, GDCh)
- International Activity
Member of the executive committee of the European Societies of
Toxicology EUROTOX Other Activities
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Letzte Aktualisierung ( Montag, 11 Oktober 2004 )
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Current Research Activities |
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Samstag, 09 Oktober 2004 |
Current Research Activities
- Regulation of MDR1 P-Glycoprotein and MRP-proteins by xenobiotics in lung cell culture
- Expression of metalloproteinases and conjugate transporters in human lung cells in culture
- Toxic and genotoxic potency of complex mixtures from plasma etching and possible interactions between components
- Role of NO-induced IGF expression with respect to the sensitivity
towards cardiotoxicants: importance for the apoptosis of myocytes
- Stability and expression of cytochrome P-450 and P-glycoproteins
in isolated perfused lungs and pneumocytes in culture
- Drug transporters
in normal tissue and tumors of the lung - role for genesis and therapy
of lung tumors
- Stability of cellular morphology of human bronchial
cells in culture and activity in metabolism and transport of
xenobiotics
- Effects of coenzyme Q10 nanodispersions in heart and lung
- Effects of heavy metals on primary cultures of human lung cells and
lung tumor cell lines
- Influence of prostaglandines on the expression of
MRPs in primary cultures of human lung cells and lung tumor cell lines
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Samstag, 09 Oktober 2004 |
Experiences
- Toxicology of halogenated hydrocarbons (DCM, DBCM, DCBM, TCM)
- Cardiovascular toxicology of halogenated hydrocarbons (telemetric measurements)
- Toxicity and genotoxicity of waste products from plasma etching
- Metabolism of tobacco smoke constituents
- Pulmonary elimination of xenobiotics
- Isolated perfused organs
- Primary culture of lung and liver cells
- Genotoxicity assays (chromosomal aberations, SCEs, HPRT assay, Comet assay)
- Chemical analysis of xenobiotics and metabolites
Methods
- Cell culture of normal human cells
- Tumor cell culture Genotoxicity assays (HPRT, SCE, Comet)
- Isolated perfused organs
- Isolation of protein, RNA and DNA
- Blotting techniques
- Functional assays (fluorimetry)
- Enzyme activities (AOEs, CYP)
- HPLC, GC, GC-MS, IC
- Real-time PCR
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Letzte Aktualisierung ( Samstag, 09 Oktober 2004 )
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weiter …
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Informationen 